anti cd68 mouse mab  (Servicebio Inc)

Journal: Cell reports

Article Title: Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency

doi: 10.1016/j.celrep.2024.114985

Figure Lengend Snippet: (A) Volcano plot of upregulated (yellow) and downregulated proteins (blue) in the thalamus of GFP- Grn −/− vs. GFP- Grn +/+ mice (fold change [FC] > 1.2, p < 0.05). (B) Bar graph of the most significantly enriched Gene Ontology (GO) terms describing the differentially expressed proteins in (A) (GFP- Grn −/− mice vs. GFP- Grn +/+ mice; FC = 1.2 and adjusted p value = 0.05). Displaying all significant changed modules ( p < 0.05). (C) Plot of PCs (PC1 vs. PC2) for GFP- Grn +/+ mice (blue), GFP- Grn −/− mice (gray), hPGRN- Grn −/− mice (purple), hGRN2- Grn −/− mice (green), and hGRN4-GFP- Grn −/− mice (yellow). Ellipses: 95% confidence interval. (D) Heatmap of top 140 proteins (rows) differentially expressed between GFP- Grn −/− and GFP- Grn +/+ and treatment groups (columns). Quantification of individual proteins shown (log 2 Z score transformed). Individual mouse numbers are below the column. (E) Bar plots comparing correction of elevated levels of LGALS3, CD68, GFAP, GPNMB, HEXB, LYZ2, MPEG1, SERPINA3N, and TPP1 in Grn −/− mice injected with GFP, hGRN2, hGRN4, or hPGRN. Mean (protein abundance) ± SD. One-way ANOVA with Tukey’s post hoc. n = 5–7 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (F) Correlation of hGRN2 (green) and hGRN4 (yellow) expression with galectin-3 (R = 0.78, p = 0.0011). (G) Correlation of hGRN2 (green) and hGRN4 (yellow) expression with P2RY12 (R = 0.77, p = 0.0014).

Article Snippet: Rabbit monoclonal anti-mouse CD68 , Cell Signaling Technology , Cat# 97778; RRID: AB_2928056.

Techniques: Transformation Assay, Injection, Quantitative Proteomics, Expressing

Journal: Cell reports

Article Title: Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency

doi: 10.1016/j.celrep.2024.114985

Figure Lengend Snippet: (A) Heatmap of differentially expressed (log 2 Z score-transformed) proteins associated with microglial activation and dysfunction (rows) in all treatment groups in GFP- Grn −/− compared to GFP- Grn +/+ (columns). (B) Abundance of CD45 (PTPRC) across all treatment groups. (C) Abundance of P2RY12 across all treatment groups. (D) Representative CD68 IHC of 12-month-old mouse coronal brain sections across all treatment groups. Scale bar: 2 mm. (E) Quantification of CD68 IHC signal of cortex, hippocampus, and thalamus. (F) Immunoblot of CD68 in cortical and thalamic brain tissue from all injection groups. (G) Quantification of immunoblot of cortical CD68 signal normalized to H3. (H) Quantification of immunoblot of thalamic CD68 signal normalized to H3. (I) Quantification of GPNMB levels in thalamus using ELISA. Data are presented as means ± SD. p values were calculated by one-way or two-way (E) ANOVA with Tukey’s post hoc analysis. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: Rabbit monoclonal anti-mouse CD68 , Cell Signaling Technology , Cat# 97778; RRID: AB_2928056.

Techniques: Transformation Assay, Activation Assay, Western Blot, Injection, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Granulins rescue inflammation, lysosome dysfunction, lipofuscin, and neuropathology in a mouse model of progranulin deficiency

doi: 10.1016/j.celrep.2024.114985

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-mouse CD68 , Cell Signaling Technology , Cat# 97778; RRID: AB_2928056.

Techniques: Recombinant, Plasmid Preparation, Virus, Staining, Blocking Assay, Hydrophilic Interaction Liquid Chromatography, Transfection, Magnetic Beads, Polymer, Avidin-Biotin Assay, Enzyme-linked Immunosorbent Assay, Software